b vectastain elite abc hrp kit cat no pk 6100 vector laboratories (Vector Laboratories)

Vector Laboratories is a verified supplier

Vector Laboratories manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Vector Laboratories b vectastain elite abc hrp kit cat no pk 6100 vector laboratories
B Vectastain Elite Abc Hrp Kit Cat No Pk 6100 Vector Laboratories, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 12758 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vectastain+elite+abc+hrp+kit+vector+laboratories+cat/VECTASTAIN+Elite+ABC+HRP+Kit+(Peroxidase%2C+Standard)/pm42003899-116-3-11
Average 99 stars, based on 12758 article reviews

b vectastain elite abc hrp kit cat no pk 6100 vector laboratories - by Bioz Stars, 2026-09

99/100 stars

Images

other:

Article Title: Antibody escape by polyomavirus capsid mutation facilitates neurovirulence
Article Snippet: DOI: https://doi.org/10.7554/eLife.61056 16 of 29 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Anti-Rat IgG AF568 (Donkey polyclonal) Abcam Cat#ab175475 IF(1:500) Antibody Anti-Rabbit IgG AF647 (Donkey polyclonal) Jackson Immuno Research Cat#711-605-152 IF(1:500) Antibody Anti-CD8a-AF700 (Rat monoclonal) Biolegend Cat#100730 FC(1:200) Antibody Anti-CD44-FITC (Rat monoclonal) Biolegend Cat#103006 FC(1:200) Antibody Anti-Rat IgG-APC (Goat polyclonal) BD Cat#551019 FC(1:200) Antibody Anti-Mouse IgG-HRP (Goat polyclonal) Biolegend Cat#405306 ELISA(1:2800) Antibody Anti-Mouse IgG-HRP (Goat polyclonal) Bethyl Laboratories INC Cat#A90-116P ELISA(1:7000) Antibody Biotinylated Anti-Rabbit (Goat Polyclonal) Vector Laboratories Cat#BA-1000 IHC(1:500) Other Mouse Polyomavirus (Strain A2) N/A N/A Strain, strain background (Escherichia coli) BL21 Agilent Cat#200133 Recombinant DNA reagent PyVP1-pGEX-4T-2 (plasmid) Provided by Robert Garcea N/A Recombinant DNA reagent H2B-GFP (plasmid) Kanda et al., 1998, Addgene Plasmid #11680 Peptide, recombinant protein Benzonase Nuclease Sigma Cat#E1014 Virus Purification (1:3333) Other Db-LT359 Tetramer NIH Tetramer Core N/A FC(1:400) Peptide, recombinant protein Neuraminidase from Vibrio cholerae (Type II) Sigma Cat#N6514 Virus Purification (1:2000) Peptide, recombinant protein RevertAid H Minus Reverse Transcriptase ThermoFisher Cat#EP0451 Chemical compound, drug OptiPrep STEMCELL Technologies Cat#07820 Chemical compound, drug Glutathione Sepharose 4B GE Healthcare Cat#17075601 Chemical compound, drug TRIzol Reagant ThermoFisher Ref#15596018 Chemical compound, drug Lipofectamine 2000 Transfection Reagent ThermoFisher Cat#11668030 Commercial assay, kit TBP PrimeTime XL qPCR Assay IDT Mm.PT.39a.22214839 Other ProLong Gold antifade reagent with DAPI ThermoFisher Ref#P36931 Other Fixable Viability Dye eFluor780 ThermoFisher Cat# 65-0865-14 FC(1:1000) Commercial assay, kit Avidin/Biotin Blocking Kit Vector Laboratories Cat#SP-2001 Commercial assay, kit VECTASTAIN Elite ABC-HRP Kit Vector Laboratories Cat#PK-6100 Commercial assay, kit NovaRED Substrate Kit Vector Laboratories Cat#SK-4800 Continued on next page Lauver, Goetschius, et al. eLife 2020;9:e61056.

Article Title: Commensal bacteria and fungi differentially regulate tumor responses to radiation therapy.
Article Snippet: In brief Depletion of commensal bacteria leads to expansion of commensal fungi and reduced antitumor immunity following irradiation of tumors.. Targeting commensal fungi enhanced the radiationinduced antitumor immune response by reducing macrophage-mediated immunosuppression.. Thus, Shiao et al. demonstrate opposing effects of commensal bacteria and fungi on antitumor immunity following radiation.

Fluorescence:

Article Title: EGFR-Pak Signaling Selectively Regulates Glutamine Deprivation-Induced Macropinocytosis.
Article Snippet: .. Compound, Sakura Finetek VWR Cat#25608-930 Dako Fluorescence Mounting Medium Agilent Tech Cat#S302380-2 PureLink RNA Mini Kit Thermo Fisher Scientific Cat#12183025 High Capacity cDNA Reverse Transcription Kit Thermo Fisher Scientific Cat#4368814 SYBR Premix Ex Taq II (Tli RNaseH Plus) TaKaRa Cat#RR820L DC Protein Assay Kit Bio-Rad Cat#5000112 NewBlot IR Stripping Buffer, 5X LI-COR Cat#928-40028 Lipofectamine RNAiMAX Transfection Reagent Thermo Fisher Scientific Cat#13778100 VECTASTAIN Elite ABC HRP Kit Vector Laboratories CatPK-6100 DAB HRP Substrate Kit Vector Laboratories Cat#PK-4100 Experimental Models: Cell Lines AsPC-1 ATCC ATCC CRL-1682 .. SW1990 ATCC ATCC CRL-2172 MIA PaCa-2 (MP-2) ATCC ATCC CRL-1420 Panc-1 ATCC ATCC CRL-1469

Reverse Transcription:

Article Title: E2F1 transcription factor mediates a link between fat and islets to promote β cell proliferation in response to acute insulin resistance.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Guinea pig anti-insulin polyclonal antibody Abcam Cat# ab7842; RRID: AB_306130 Insulin (H-86) antibody Santa Cruz Biotechnology Cat# sc-9168; RRID: AB_2126540 anti-bromodeoxyuridine, anti-BrdU antibody Dako Cat# M0744; RRID: AB_10013660 Rabbit anti-Mouse CENP-A IF Preferred Monoclonal Antibody Cell Signaling Technology Cat# 2048; RRID: AB_1147629 Rabbit anti-Akt Antibody Cell Signaling Technology Cat# 9272, RRID:AB_329827 Rabbit anti-Phospho-Akt (Ser473) Antibody Cell Signaling Technology Cat# 9271, RRID:AB_329825 Rabbit anti-p44/42 MAPK (Erk1/2) Antibody Cell Signaling Technology Cat# 9102; RRID: AB_330744 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP(tm) Rabbit mAb antibody Cell Signaling Technology Cat# 4370; RRID: AB_2315112 Rabbit Anti-FoxO1 antibody Cell Signaling Technology Cat# 2880; RRID: AB_2106495 Rabbit Anti-E2F1 antibody Cell Signaling Technology Cat# 3742; RRID: AB_2096936 Alpha-tubulin antibody [DM1A] Abcam Cat# ab7291; RRID: AB_2241126 Donkey Anti-Goat IgG H&L (Alexa Fluor 488) Abcam Cat# ab150129; RRID: AB_2687506 Alexa Fluor 594 AffiniPure Donkey Anti-Guinea Pig IgG (H+L) Jackson ImmunoResearch Cat# 706-585-148; RRID: AB_2340474 Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 Invitrogen Cat# A-21206; RRID: AB_141708 Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 555 Invitrogen Cat# A-31572; RRID: AB_162543 Bacterial and virus strains Lentiviral particles for control scramble shRNA Santa Cruz Biotechnology Cat#sc-108080 Lentiviral particles for murine E2F1 shRNA Santa Cruz Biotechnology Cat#sc-34257-v Biological samples Human islets, see Table S2 Alberta Islet Distribution Program, University of Alberta ADI Islet Core N/A Chemicals, peptides, and recombinant proteins DAPI, dilactate Sigma-Aldrich Cat#D9564 S961 Lauge Schaffer (Novo Nordisk) (Schaffer et al., 2008) N/A OSI-906 Med Chem express LLC Cat#HY-10191 5-Bromo-20-deoxyuridine Sigma-Aldrich Cat#B9285 Miami Medium #1A Cellgro Cat#98-021-CV Final Wash/Culture Medium Cellgro Cat#99-785-CV Polybrene Santa Cruz Biotechnology Cat#sc-134220 M-PERTM Mammalian Protein Extraction Reagent Thermo Fisher Scientific Cat#78501 Protease Inhibitor Cocktail Sigma-Aldrich Cat#P8340 Phosphatase Inhibitor Cocktail 2 Sigma-Aldrich Cat#P5276 Phosphatase Inhibitor Cocktail 3 Sigma-Aldrich Cat#P0044 PierceTM BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23225 (Continued on next page) e1 Cell Reports 41, 111436, October 4, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER RNeasy Mini Kit QIAGEN Cat#74106 High-Capacity cDNA Reverse Transcription Kit Thermo Fisher Scientific Cat#4368813 SYBR Green Supermix Bio-Rad Cat#1725274 VECTASTAIN Elite ABC HRP Kit Vector Laboratories CatPK-6101 HLM 006474 Tocris Cat#5283 Critical commercial assays Ultra Sensitive Mouse Insulin ELISA Kit Crystal Chem Cat#90080 Contour Blood Glucose Meter Bayer Health Care Cat#9545C CellTiter 96 Non-Radioactive Cell Proliferation Assay (MTT) Promega Cat#G4001 Click-iT Plus EdU Alexa Fluor 488 Imaging Kit Thermo Fisher Scientific Cat#C10637 Click-iT Plus EdU Alexa Fluor 594 Imaging Kit Thermo Fisher Scientific Cat#C10639 Ultra Sensitive Mouse Insulin ELISA Kit Morinaga Institute of Biological Science, Yokohama, Japan Cat#MS303 Glutest Neo Super Sanwa Chemical Co. Kanagawa, Japan N/A Deposited data Microarray data, see Table S1 This paper N/A Experimental models: Cell lines Control b-cells Laboratory of R. N. Kulkarni (Assmann et al., 2009; Kulkarni et al., 1999) N/A IRS1KO b-cells Laboratory of R. N. Kulkarni (Assmann et al., 2009; Kulkarni et al., 1999) N/A IRS2KO b-cells Laboratory of R. N. Kulkarni (Assmann et al., 2009; Kulkarni et al., 1999) N/A b-cell-specific IR KO (bIRKO)-cells Laboratory of R. N. Kulkarni (Assmann et al., 2009; Kulkarni et al., 1999) N/A sh-CENPA Control b-cells Laboratory of Jun Shirakawa (Shirakawa et al., 2017b) N/A sh-E2F1 Control b-cells This study N/A sh-scramble Control b-cells This study N/A Experimental models: Organisms/strains bIRKO mouse Laboratory of R. N. Kulkarni (Kulkarni et al., 1999) N/A IR-floxed mouse Laboratory of R. N. Kulkarni (Kulkarni et al., 1999) N/A IRS-2 knockout mouse Laboratory of Yasuo Terauchi (Kubota et al., 2000) N/A Oligonucleotides Primers for qPCR, see Table S3 This paper N/A Software and algorithms ImageJ software NIH https://imagej.nih.gov/ij/ Prism 8 software Graph Pad Software https://www.graphpad.com/ scientific-software/prism/ BIOREVO software KEYENCE https://www.keyence.co.jp/ Cell Reports 41, 111436, October 4, 2022 e2 ..

DC Protein Assay:

Stripping Membranes:

Transfection:

Article Title: Pre-clinical safety and efficacy of human induced pluripotent stem cell-derived products for autologous cell therapy in Parkinson's disease.
Article Snippet: Clinical and Translational Report

Polymerase Chain Reaction:

RNA Sequencing:

Whole Complete genome sequencing:

Mouse Assay:

Real-time Polymerase Chain Reaction:

SYBR Green Assay:

Article Title: Melanocyte Stem Cell Activation and Translocation Initiate Cutaneous Melanoma in Response to UV Exposure.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Donkey anti-rabbit IgG (H&L) antibody peroxidase conjugated Rockland Cat#611-703-127; RRID: AB_218614 Donkey anti-rat IgG (H&L) antibody peroxidase conjugated Rockland Cat#612-703-002; RRID: AB_219797 Donkey anti-mouse IgG (H&L) antibody peroxidase conjugated Rockland Cat#710-703-124; RRID: AB_217688 Bacterial and Virus Strains One Shot Stbl3 Chemically Competent E. Coli Invitrogen Cat#C737303 Chemicals, Peptides, and Recombinant Proteins Doxycycline hyclate Alfa Aesar Cat#J60579 Tamoxifen Sigma-Aldrich Cat#T5648 4-Hydroxytamoxifen Sigma-Aldrich Cat#T176 Phorbol 12-myristate 13-acetate AdipoGen Cat#A00083/B Dexamethasone TCI America Cat#D1961 Esp3I ThermoFisher Scientific Cat#ER0451 DTT Amresco Cat#0281-25G Deoxynucleotide Mix G-Biosciences Cat#786-442 Taq DNA Polymerase G-Biosciences Cat#786-447 Ethidium Bromide Amresco Cat#X328-10mL Matrigel Matrix Basement Membrane Corning Cat#354234 Puromycin dihydrocholoride, 99% Alfa Aesar Cat#J61278 Penicillin-Streptomycin Thermo Fisher Scientific Cat#15140122 Collagenase type I GIBCO Cat#17100-017 Collagenase type IV GIBCO Cat#17104-019 TRIzol LS Invitrogen Cat#10296028 Lipofectamine 3000 Invitrogen Cat#L3000-001 Isoflurane Piramal Healthcare Cat#NDC66794-013 NairTM Church & Dwight Critical Commercial Assays Rapid DNA Ligation Kit Thermo Fisher Scientific Cat#K1422 EZNA Plasmid Midi Kit Omega Bio-Tek Cat#D6904 PureLink Genomic DNA Mini Kit Invitrogen Cat#K1820-01 SuperScript II First-Strand Synthesis SuperMix Invitrogen Cat#18080-400 PerfeCTa SYBR Green SuperMix Quanta BioSciences Cat#95054-100 VECTASTAIN Elite ABC HRP Kit Vector Laboratories CatPK-6100 ImmPACT AEC Peroxidase (HRP) Substrate Vector Laboratories Cat#SK-4205 BCA assay Thermo Fisher Scientific Cat#23227 SuperSignal West Pico chemiluminescence Thermo Fisher Scientific Cat#34087 NEBNext Ultra Directional RNA Library Prep Kit New England BioLabs Cat#E7420 Qubit dsDNA HS Assay Kit Thermo Fisher Scientific Cat#Q32851 Mouse Cytokine Antibody Array C3 RayBiotech Cat#AAM-CYT-3-8 Deposited Data RNaseq data This paper GEO: GSE102597 Experimental Models: Cell Lines .. Murine melanoma cell This paper N/A Experimental Models: Organisms/Strains NOD-scid IL2Rgnull Jackson Laboratories Stock#: 005557 B6.Cg-Tg(Tyr-cre/ERT2)13Bos/J Jackson Laboratories Stock#: 012328 (Continued on next page) e2 Cell Stem Cell 21, 1–14.e1–e6, November 2, 2017

Enzyme-linked Immunosorbent Assay:

Proliferation Assay:

MTT Assay:

Imaging:

Microarray:

Control:

Knock-Out:

Software:

Virus:

Recombinant:

Article Title: Osteopontin associates with brain T RM -cell transcriptome and compartmentalization in donors with and without multiple sclerosis.
Article Snippet: ll OPEN ACCESS iScience

Membrane:

DNA Ligation:

Plasmid Preparation:

BIA-KA:

Ab Array:

RNA sequencing:

Blocking Assay: